Journal: Investigative Ophthalmology & Visual Science
Article Title: Effect of Corneal Nerve Ablation on Immune Tolerance Induced by Corneal Allografts, Oral Immunization, or Anterior Chamber Injection of Antigens
doi: 10.1167/iovs.16-20601
Figure Lengend Snippet: Effect of trephining on integrity of corneal nerves. ( A ) Corneas of the right eyes of BALB/c mice were trephined and the corneas of both eyes were removed 2, 4, 20, and 50 days later and were stained with rabbit anti-mouse β III tubulin primary antibody. ( B ) Corneal nerve density was quantified using ImageJ analysis. ( C ) The anterior segment (cornea, iris, and ciliary body) of the trephined or unmanipulated contralateral eye was excised and assessed by q-PCR for NK1-R message at the times indicated. The results represent the mean ± SEM (5 mice/group). Results were similar in two additional experiments.
Article Snippet: Corneas were incubated with rabbit anti-mouse β III tubulin primary antibody (TUJ1) IgG (Covance, Richmond, CA, USA), washed in PBS, and incubated with propidium iodide and secondary antibody (Alexa 488 goat anti-rabbit IgG; Invitrogen, Carlsbad, CA, USA) for 2.5 hours at room temperature.
Techniques: Staining